The Study on the Effect and Mechanism of Crocin against Human Ewing Sarcoma

Ewing sarcoma is the second most common primary malignant bone tumor in pediatrics after osteosarcoma, which mainly occurs children and adolescents aged 5-20 y. Crocin main active ingredient extracted from Crocus sativus has significant inhibitory effect on many cancer cells. Therefore, this study observed of crocin human its specific mechanism provided molecular theoretical basis for clinical application. We cultured Human cells lines SK- ES-1 RD-ES vitro treated them with 0, 2.5, 5 10 mmol/l solution. And we used cell counting kit-8 assay to detect viability adopted flow cytometry apoptosis cycle changes, western-blot expression apoptotic pathway-related proteins. At same time, also combined vincristine treat cells, apoptosis, observe synergistic effect. established xenografts model Ewing’s nude mice xenografts. could not only inhibit growth SK-ES-1 a dose-dependent manner, but increase block progression. The results analysis showed that significantly increased degradation poly adenosine diphosphate-ribose polymerase caspase-3, levels anti-apoptotic proteins B-cell lymphoma 2, lymphoma-extra-large p21. proliferation inhibition sarcoma. either alone or combination vincristine. This was mediated by increasing apoptosis-related pathways.